Enzymes and Inhibitors
New England Biolabs, Inc. PstI – 10000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.
New England Biolabs, Inc. PflMI – 1000 units
A restriction endonuclease that recognizes the sequence CCAN_NNN^NTGG.
New England Biolabs, Inc. I-SceI – 2500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. BseYI – 100 units
A restriction endonuclease that recognizes the sequence C^CCAG_C.
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 500U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
New England Biolabs, Inc. BstEII-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GTNACC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. AflII – 10000 units
A restriction endonuclease that recognizes the sequence C^TTAA_G.
Cayman Chemical BAM-22P8-22humnmouse1mg
A neuropeptide and MRGPRX1 agonist; induces calcium mobilization in HEK293S cells expressing human MRGPRX1 or SNSR3 (EC50s = 14 and 28 nM, respectively); inhibits voltage-induced calcium currents in isolated rat neurons expressing human MRGPRX1 (EC50 = 0.6 µM); intrathecal administration decreases the tail-flick latency in pertussis toxin-stimulated rats and increases the tail-flick latency in U-73122-stimulated rats, indicating changes in pain sensitivity, at 30 nmol/animal; increases scratching behavior in wild-type mice and enhances bile-duct ligation-induced scratching behavior in a mouse model of cholestasis
New England Biolabs, Inc. Endonuclease V – 250 units
Endonuclease V is a repair enzyme found in E. coli that recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA. Endonuclease V, often called deoxyinosine 3 endonuclease, recognizes DNA containing deoxyinosines (paired or not) on double-stranded DNA, single-stranded DNA with deoxyinosines and to a lesser degree, DNA containing abasic sites (ap) or urea, base mismatches, insertion/deletion mismatches, hairpin or unpaired loops, flaps and pseudo-Y structures. It is believed that Endonuclease V needs another protein to repair the DNA, as it does not remove the deoxyinosine or the damaged bases. Endonuclease V cleaves the second phosphodiester bonds 3' to the mismatch of deoxyinosine, leaving a nick with 3'-hydroxyl and 5'-phosphate.
New England Biolabs, Inc. BclI-HF – 3000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. SfiI – 15000 units
A restriction endonuclease that recognizes the sequence GGCCN_NNN^NGGCC.
New England Biolabs, Inc. NlaIII – 500 units
A restriction endonuclease that recognizes the sequence _CATG^.
New England Biolabs, Inc. ScaI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. T7 Endonuclease I – 250 units
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.
New England Biolabs, Inc. Tsp45I – 200 units
A restriction endonuclease that recognizes the sequence ^GTSAC_.